A simple, rapid, accurate and sensitive UV-spectrophotometric method has been developed and validated successfully for the estimation of mifepristone in bulk and in-house formulation. Using, zero order spectrum of mifepristone was derivatized into first order by software UV Probe 2.21. ‘Method A’ is for zero order derivative and ‘Method B’ is for zero order area under curve (AUC) technique; measured in the wavelength set from 287.00-318.80 nm. While ‘Method C’ is for 1st order derivative and ‘Method D’ is 1st order AUC technique; was measured in the wavelength set from 310.00-338 nm, in which area under curve was integrated in the wavelength set at 323.60 nm. The λmax of mifepristone in methanol was found to be 305 nm. The drug shows linearity in the range from 3-18 µg/ml with r2 value was 0.998. The current method was introduced for qualitative and quantitative estimation of mifepristone in bulk and in-house formulation. This developed method can be used for routine analysis.
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